伝染性造血器壊死症ウイルスの持続感染細胞〔英文〕

概要

論文の詳細を見る
Persistent infection in YNK cells derived from Yamame Oncorhynchus masou kidney with a non-pathogenic variant of infectious hematopoietic necrosis virus (IHNV) was established. The virus used to initiate persistent infection was isolated from rainbow trout Salmo gairdneri. Indirect fluorescent antibody studies showed that the persistently infected cells, YNK (SE-IHNVL), had similar antigenic properties to wild-type IHNV. YNK (SE-IHNVL) cells are presumably infected with a temperature-sensitive mutant of IHNV, because of a normal cell appearance and multiplication occur at 20°C but cytopathic effect (CPE) and destruction of the cells follows incubation at 15°C. YNK (SE-IHNVL) cells produce the virus (HS7912 IHNV) with weak cytopathogenicity on tissue culture cells constantly (103.8-104.8 50% tissue culture infective dose perml). This virus destroys cell monolayers at 15°C but not at 20°C. The infection of HS7912 IHNV was neutralized by anti-wild-type IHNV serum. HS7912 IHNV had so little pathogenicity against rainbow trout fry that only 1% of inoculated fish were killed, while the mortality of fish infected with IHNV was 40%. By superinfection study, HS7912 IHNV was found to infect tissue and to interfere with the infection of wild-type IHNV.
日本水産學會の論文