溶菌酵素生産菌の細胞自己溶解〔英文〕

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The autolysis of bacterial cells (strain V37) capable of producing lytic enzyme was investigated using the washed cells gown in polypepton-yeast extract medium. Strain V37 cells grown in the medium containing 0.5M NaCl or KCl were conspicuously auto-lysed than those grown in the medium comprising 0.5M MgCl2 or without addition of any in-organic salt. The optimum pH and temperature for the autolysis of strain V37 cells were 7 to gand 40°C, respectively. The autolytic activity slightly increased in the presence of 0.2-0.3M NaCl in 0.05M Tris-HCl buffer (pH 7.0), but was significantly inhibited by bivalent cations such as Ba2+, Ni2+, Co2+ and Mg2+. The presence of NaCl (0.4-0.9M) in the medium, enhanced then ability to autolyse.
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