Studies of digitalis (Report 1)
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The author has come to the following conclusion from the observations of time-concentration curves after subcutaneous injections of active principles of <I>digitalis purpurea</I> in the aqueous solution, as the dose of 1/40 (cc) body weight of <I>Rana nigromaculata</I> at the room temperature of 25°C. (1) The variations of the potency of leaves, dried either with sunlight or with infrared ray, were almost the same to each other. (2) The method of extraction of digitalis powder from leaves dried was proved more excellent than that from leaves without any treatment. (3) There were far less amount of glycosides contained in flowers than iu leaves. (4) In comparison of the amount of active principles of digitalis dried the newer leaves were the richest in amount, according to the length of the preservation period. (5) The difference in activity between the same species of digitalis ranged in one to four, according to thier cultivating location. (6) The method, in which the active principles have been extracted from the leaves in the cold infusion with methanol at first and then with ethanol as the first step of purification, seemed as the most excellent. (7) The principles extracted from leaves with ethyl acetate exerted a rapid effect, which appeared to contain genins as a main constituent. (8) The principles with a slow effect, contained in the aqueous solution of the extract, was strongly destroyed by acid, and all of the active principles were deprived of their effectiveness by alkali. (9) The active principles with rapid effect existing in the leafpowder or dried extracts were more labil than that with slow effect under the 'humidity in room and by the sunlight, but when kept with the leafpowder it was comparatively stable. (10) The aqueous solution of extracts prepared by methanol, was hardly changed in its effect, even if it was heated up to 95°C for half an hour. (11) Butoben and 1 per cent of the aqueous solution of glucose were of use to making glycosides stable, while glycerol and alcohol were little of use, and propylene glycol was rather harmful against the digitalis action. (12) Most of the commercial preparations of digitalis contained chiefly the active principles with rapid effect and their potency inclined to be overestimated. (13) When the feature of ventricular arrest of frog's heart were taken into consideration, digitoxin was proved as the cardiotonic with the most slow effect and k-strophanthin was more slower than the purpurea glycosides in effect. (14) The ratio of toxic dose to the effective dose was smaller in <I>k</I>-strophanthin than in digitoxin, where the cardiotonic effect of digitoxin and raw extract of digitalis appeared earlier than k-strophanthin.
- 社団法人 日本薬理学会の論文
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関連論文
- A experimental criticism about four-hours method as bioassay of digitalis
- Studies of digitalis (Report 1)