Tetraphenylphosphonium (TPP<SUP>+</SUP>) によるシナプトソームの膜電位の測定

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概要

• 論文の詳細を見る

• The concentration of tetraphenylphosphonium (TPP<SUP>+</SUP>) was measured under various controlled conditions with a TPP-selective electrode. Assuming that membrane potential in synaptosomes is depolarized replacement of sodium with potassium in the physiological saline, we estimated the amount of TPP bound to plasma membranes and mitochondria in synaptosomes. About 50% of TPP<SUP>+</SUP> was bound to plasma membranes and about 45% was mitochondria in synaptosomes. Membrane potentials in synaptosomes under various conditions were calculated by the Nernst equation using these values. The resting potential of synaptosomes was estimated to be-70 to-90mV.<BR>The propriety of these values obtained and of the method, for calculation were discussed.

• 日本膜学会の論文

著者

• 松永 雅美

  Laboratory Of Biological Chemistry School Of Pharmaceutical Sciences Showa University

• 松永 雅美

  昭和大学教養部生物学教室

• 相内 敏弘

  学部生物化学教室

• 中谷 一泰

  学部生物化学教室

• 高橋 剛男

  昭和大学教養部生物学教室

• 中村 泰治

  学部生物化学教室

関連論文

• Calculation of Membrane Potential in Synaptosomes with Use of a Lipophilic Cation (Tetraphenylphosphonium)
• Tetraphenylphosphonium (TPP+) によるシナプトソームの膜電位の測定

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