Rapid Isolation and Identification of Individual Microvillous Proteins from a Nitrocellulose Membrane Filter

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概要

• 論文の詳細を見る

• Several organic solvents were used to dissolve the nitrocellulose filter to which proteins were bound. Among these solvents, acetone and methanol easily dissolved the nitrocellulose. Proteins released from the nitrocellulose were precipitated by centrifugation. The efficiency of protein recovery was investigated using commercially available proteins. In this study, we found that a 50% acetone-50% methanol was most effective to recover the intestinal microvillous membrane proteins from a nitrocellulose filter. Subsequent digestion of these proteins with CNBr, or some proteinases, exposed internal fragments and enabled their microsequencing.

• 日本膜学会の論文

著者

• Uezato Tadayoshi

  Department Of Biochemistry Hamamatsu University School Of Medicine

関連論文

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• Rapid Isolation and Identification of Individual Microvillous Proteins from a Nitrocellulose Membrane Filter

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