Pathogenicty of the Nonphotochromogenic Strains of Unclassified Mycobacteria Isolated from Dogs for Mice and Dogs
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Fifteen strains of mycobacteria have been isolated by the present authors from the lymphnodes of dogs without owners. Studies on the cultural and staining characteristics, biochemical properties (Niacin and urease test), drugsensitivity, phage-susceptibility, specificity in tuberculin reaction and pathogenicity for guineapigs and chickens have disclosed that these mycobacteria were classified into three groups. Seven strains of the mycobacteria (group A) were identified as human type tubercle bacilli, six strains (group B) as the nonphotochromogens and the remaining two s-trains as "rapidgrowers".<BR>The purpose of the present paper is to report the pathogenicity of the nonphotochromogenic strains of group B for mice and dogs.<BR>Materials and methods<BR>Nonphotochromogenic strains of group B used were Kasuya 24, Mizuma 21-2, Ukiha 1-1, Ukiha 1-2, Yakatabaru 3. Besides these the following strains were used as controls: <I>Mycobacterium tuberculosis</I> (H37Rv strain), <I>M. avium</I> (3717 strain), a photochromogen (P16 strain), two nonphotochromogens (100616 and Gamou strains).<BR>Animals used were male white mice (ddn strain) and young dogs without owners. Inoculums were prepared from the two week-culture of each strain on Ogawa's egg medium. One tenth ml. of 5 mg. per ml of bacterial suspen sion was inoculated intravenously into mice and a half ml. of 20 mg. per ml. of the suspension intraperitoneally into dogs. The actual infecting doses were checked by viable count. Viable counts of infecting organisms in various organs of infected animals were carried out at various periods after inoculation.<BR>Tuberculin tests were performed on infected dogs 4 and 8 weeks after infection. Each 30μg. of purified tuberculin protein π prepared from culture filtrates of the H37Rv, Mizuma 21-2 and Kasuya 24 strains were injected in tracutaneously and the diameters of indurations were measured after 48 hours.
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