抗酸菌のL相をめぐつて : 1. 抗酸菌の細胞壁欠損型
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概要
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Peculiarity of tuberculosis is a fact that a huge amount of living but invisible tubercle bacilli are contained in caseous lesions. Such a survival form of tubercle bacillus had been suggested by pathologists through their past studies. This survival mode of tubercle bacillus is comparable with the L form of other microbial species. The author intends to review studies on Cell Wall Deficient Forms, including L form, of tubercle bacillus <I>in vitro</I>.<BR>In the present paper, it is proposed to classify Cell Wall Deficient Form into two groups, multiplying and non-multiplying, the former being L form and the latter spheroplast or protoplast. Spheroplast and protoplast were produced by application of lysozyme or bacteriophage on the classical form of Mycobacteria. A strain of <I>M. smegmatis</I> converted nearly completely into its spheroplast in the modified Trypto -Soy broth containing suitable inducers, and its metabolizing activity was confirmed.<BR>Various kinds of media had been employed for producing the L form of tubercle bacillus. The author observed the appearance of L form of Mycobacteria from several species employing modified Trypto-Soy Agar medium: 3A and 3B type colonies of <I>M. smegmatis</I>, 3A type colonies of <I>M. tuberculosis</I> and 3A type colonies of<I> M. avium</I> were isolated from the classical form of each species. The 3A colony of the L form consisted of spherules sized from 100nm to 30μm without any special structure of Mycobacteria under electron microscope, while the 3B type colonies consisted of spherules containing the inside structures resembling to those of classical form of Mycobacteria.<BR>The above described findings suggested an easy r eappearance of classical form of Mycobacteria from 3B colonies and rather retard reversion of Mycobacteria from 3A colonies. The L form seems to be a form for the survival of Mycoba cteria <I>in vivo</I> to continue endless multiplication with minimal, metabolizing activity, thus avoiding from the immunological activities of the host.
- 日本結核病学会の論文