非定型抗酸菌Battey菌感染症の2例

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Two strains of mycobacteria were isolated from two patients (Okada and Okutsu) who had been treated as pulmonary tuberculosis cases. These strains were considered as causes of infections of these patients, because of the frequencies and numbers of colonies of isolations. In order to identify these strains of mycobacteria, various biochemical and biologica l characteristics, and pathogenicities to mice and rabbits were investigated. The cultures used in this study were the Okada strain and the Okutsu strain which had been isolated from sputa of these patients, and as the control, the Okayama strain of Mycobacterium (M.) intracellula re, M. terrae 1722, M. gastri W-417 and the T-435-5 strain of Subgroup “V”of Group III nonphotochromogens, the Kirchberg strain of M. avium, M. tuberculosis, H37Rv and M. bovis, Ravenel were used. Biochemical and biological characteristics of the Okada strain and the Okutsu strain were as follows: Colonies of both strains on 1% Ogawa egg medium and 7 H 10 medium were nonphotochromogenic and smooth. Two organisms gave negative cord formation and neutral red test. Growth was observed at 22°C, 33°C and 37°C in both strains, and besides at 42°C in the Okada strain. Both organisms were susceptible to O.1 M of sodium nitrite and 1, 000 mcg/ml of PAS, whereas resistant to 500 mcg/ml hydroxylamine, 1.0% sodium desoxycholate, 0.05%pyronin and 10 mcg/ml TCH. Both organisms gave negative niacin test, negative nitrate reductase, weakly positive semiquantitative catalase, positive 68°C heat stable catalase, positive acid phosphatase and negative 70°C heat stable acid phosphatase. Three day arylsulfatase test was negative in both organisms, and two week and three week arylsulfatase tests were positive in the Okada strain but negative in Okutsu strain. Amidases of 12 amides were all negative in both organisms. Of 16 carbohydrates, glucose, trehalose, mannose and fructose were decomposed by both organisms, and besides galactose by the Okada strain. Both organisms utilized sodium (sod.) acetate, sod. pyruvate, sod. propionate, sod. lactate and sod. fumarate in 10 organic acid salts. Pathogenicities of the Okada strain and the Okutsu strain to mice and rabbits were as follows: In mice infected with the Okada strain, tubercles were found macroscopically in the lungso f eightm icew hichw ereo bserve4d to 6 weeksa fter infectiona, n d in the s plJenso f two mice which were observed at 6th week. The number of viable units from lungs, livers, kidneys and spleens was extremely large, and increased from 2nd week to 6th week. In mice infected with the Okutsu strain, no tubercle was found and the number of viable units was relatively small. In two rabbits infected with the Okada strain and the Okutsu strain, viable units from blood cultures at 7th day and 14th day, and those from visceral organs at 15th day were extremely smaller in number than those in the rabbits infected with the Kirchberg strain of M. avium as the control which died from sepsis of Yersin type at 15th day. From many biological and biochemical properties above mentioned, we could d ifferentiate the Okada strain and the Okutsu strain from M. terrae, M. gastri, Subgroup “V”, M. tuberculosis and M. bovis, but could not find out any different properties between these two strains and M. intracellulare or M. avium. However, from the point of pathogenicityto rabbits, the present two strains isolated may not be M. avium but be M. intracellulare.
日本結核病学会の論文

非定型抗酸菌Battey菌感染症の2例

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