実験的肺肉芽腫におけるアンギオテンシン転換酵素の局在と経時的推移 : Substrate film法による新しい試み

概要

論文の詳細を見る
Localization of ACE (angiotensin-converting enzyme) and its activity in the experimental pulmonary granuloma of rabbit were studied by ACE substrate film prepared from hippuryl L-hystidyl-L-leucine and purified calfskin gelatin. The pulmonary granulomas were produced in the rabbits by intravenous injection of 1.0ml of Freunds complete adjuvant or 40mg of Japanese strain of Bacillus Calmette-Guerin (BCG). At 5, 14, 21, 28, 35, 42, and 49 days after injection, the resulting pulmonary lesions (granulomas) were obtained from the rabbits exsanguinated by severing the femoral artery. Serum ACE activities were also examinated at each period.<BR>The granulomas produced by Freunds complete adjuvant at 21 and 28 days of age showed high ACE activity. The activity was seen as numerous sharply defined vacuoles on the substrate film under the epithelioid cells. Those of 42 days of age showed low activity. On the other hand, the granulomas produced by BCG showed generally low ACE activity and the activity was only seen as poorly defined and poorly stained plaques in the granulomas at 14 day of age. The serum ACE levels and the tissue ACE activities changed in a same way through the experi mental course.<BR>These findings show that ACE substrate film technique is available for determination of localization and activity of ACE and that most of increased serum ACE in this experiment may originate from tissue ACE in the granulomas.