A BACTERIOLOGIC STUDY OF THE LESIONS RESECTED FROM PULMONARY TUBERCULOSIS PATIENTS
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概要
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A bacteriologic study was made on 113 specimens of surgically removed lesions from 52 pulmonary tuberculosis patients.<BR>These lesions were aseptically incised, and the contents were transferred directly, without NaOH treatment, on Ogawa's egg medium for the cultivation of <I>M. tuberculosis.</I> Five specimens of caseous materials from which the detection of <I>M. tuberculosis.</I>by cultivation might not be successful were also inoculated into guinea pigs. With some samples, cultivation of bacteria other than <I>M. tuberculosis.</I>and of fungi were also attempted in order to detect probable mixed infection. Smears were stained, usually, by the combined staining method of Ziehl and Heidenhain (Uyeda), and the number of acid-fast forms and that of easily-stainable forms (Uyeda) were determined.<BR>Results were summarized as follows:<BR>1. The number of colonies of <I>M. tuberculosis.</I>does not always coincide with the number of acid-fast forms, but agrees approximately with the number of easily stainable forms. We can well understand these findings if we take into consideration the opinion of Uyeda that the easily-stainable form must be the viable form of <I>M. tuberculosis.</I> In some particular cases, however, the easily-stainable forms were demonstrated but the finding of the cultivation was negative.<BR>2. Though the smears were positive for acid-fast forms, the cultures were often negative even with the materials from the cavities or the liquefied lesions, especially small circumscribed lesions of the cases which had been treated with artificial pneumothorax.<BR>3. The clear-cut difference in the morphology and the viability of tubercle bacilli from the patients treated and not treated with chemotherapeutics was not recognized.<BR>4. Pulmonary tuberculous lesions are, in almost all cases, free from mixed infection.5. The previous treatment of pathogenic materials with NaOH for the cultivation of <I>M. tuberculosis.</I>remarkably decreased the number of colonies. Therefore, materials which were aseptically removed, for example, surgically resected pulmonary lesions, should be cultured without the preliminary treatment of NaOH.
- 一般社団法人 日本結核病学会の論文