STUDIES ON VOLE BACILLUS (WELLS):VI. ON THE VOLE TUBERCULIN
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概要
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VOLE bacillus (murin type of <I>M. tuberculosis</I>) strain D15 was cultured on Sauton's synthetic medium. After each of eight, ten and twelve weeks cultivation, the culture filtrate was concentrated to 1/10 of the original volume by evaporation. The concentrated filtrate was used as the original solution of crude vole tuberculin. Ortho-amino-azo (O-A-Azo) vole tuberculin was also prepared from a part of the original solution following H. Okamoto and S. Koshimura's method. The crude vole tuberculin and the O-A-Azo vole tuberculin were compared with the respective preparations of the culture filtrate of human type <I>M. tuberculosis</I>, Aoyama, B, i. e. the standard old tuberculin and the O-A-Azo old tuberculin.<BR>There was no significant difference among the vole tuberculins prepared from three different culture ages. Their potency was found equivalent each other both on human and on animal tests, all being nearly half of the potency of old tuberculin.<BR>Lower potency of vole tuberculin may due to the smaller amount of active principle of tuberculin produced in Sauton's medium on which the growth of vole bacillus was distinctly dysgonic. It was interesting to note that the sensitization of guinea pigs with heat-killed vole bacillus suspended in paraffin il was stronger than that of Aoyama B.
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