多くの施設で作つた小川培地の結核菌分離性能についての観察

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Not only in the isolation of tubercle bacilli from specimens but also in the direct drug sensitivity test, 3% Ogawas whole egg medium which contains KH2PO4 in a concentration of 3% is most widely used in Japan. Because, it is generally recognized that this medium is superior to Löwenstein-Jensen medium both in the simplicity in construction and in the practical usability. However, concerning the uniformity of the efficiency of all media prepared in different laboratories no observation has been made yet. On the occasion of the Tuberculosis Mass-survey carried out in 1963 in Japan each 10 slants of 3 % Ogawas medium prepared in 47 different local Public Health laboratories were collected to the National Institute of Health laboratory to make the comparative studies on the efficiency in the isolation of tubercle bacilli. Number of slants tested was 149 in total including each 3 slants of 3 different batches of media prepared in NIH laboratory. Two sputum specimens from tuberculosis patients were homogenized strongly with equal volume of 2% NaOH solution employing homogenizer and were diluted ten-fold with 2% NaOH. Two dilutions of 10-3 and 10-4 of one sputum and a dilution of 10-3 of the other were. inoculated onto 149 slants of 3% Ogawas media by three technicians. From the former sputum (a) the growth of tubercle bacilli appeared after 4 weeks incubation, but from the latter (b) it was after 6 weeks as shown in Tables 1 and 3. Square root of the average colony number developed on 149 slants was 5.60. Table 2 shows the statistical analysis of the variance of the colony number listed in. Table 1. According to the colony number these different 50 batches of media samples were grouped into 3, ie, A, B and C. To the group A 39, to B 3 and to C 8 batches belonged respectively as shown in Fig. 1. Square root of the average colony number in each group was 5.73, 6.78 and 4.60 respectively. In each group the difference was statistically insignificant between different batches. But, the differences between group A and B, and group A and C were highly significant. That the average number of group B was larger than that of group A may be effected by the rather larger content of the condensed water in slants. Contrary to this, the smaller mean number of group C than group A may be due to some special conditions in the process of media preparation. The latter sputum (b) gave the results that growth of tubercle bacilli was greatly affected by the type of stopper on the slants; namely, retarded growth or no growth was observed on the slants with air passable rubber stopper. From these observation it was shown that 3% Ogawas media prepared in different laboratory in Japan are rather uniform and not so much different in the efficiency of isolating tubercle bacilli from specimens.

多くの施設で作つた小川培地の結核菌分離性能についての観察

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