コレラ菌由来伝達性Rプラスミッド

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概要

• 論文の詳細を見る

• This report deals with the genetic and molecular characteristics of four transferable R plasmids isolated from Vibrio cholerae (biotype El Tor) which was isolated in Nigeria and East Asia in 1977 and 1978, respectively.1) The R plasmids conferred a resistance to all or some of chloramphenicol (CP), tetracycline (TC), streptomycin (SM), sulfonamide (SA), kanamycin (KM) and ampicillin (ABPC) on the host bacteria. The levels of resistance conferred by them were high to SM, KM, SA, and ABPC, moderate to CM, and markdely low to TC.2) All the R plasmids tested were conjugative. Their frequencies of transfer per donor by 2-hour conjugation were approximately from 10-4 to 10-5 for pJT-17 pJT-45, and from 10-2 to 10-3 for pJT-12 and pJT-13.3) The plasmids were non-thermosensitive in both transferability and replication.4) The plasmids belonged to the incompatibility C group.5) The plasmids were stable in both Vibrio cholerae and Escherichia coli even after ten subcultures in drug-free media.6) The molecular weights of CCC DNA plasmids, pJT-17 and pJT-45, were approximately 90 and 99 megadaltons, respectively, and those of pJT-12 and pJT-13 were both 106 megadaltons.7) Two R plasmids, pJT-17 and pJT-45, showed a temporary but dramatic increase (10, 000-fold) in transfer frequency during the logarithmic phase, as compared with the stationary phase. This phenomenon during the logarithmic phase was accompanied with the formation of multiple sex pili on the surface of Escherichia coli negative for general pili.

• 日本細菌学会の論文

著者

• 加納 堯子

  順天堂大学

• 加納 堯子

  順天堂大学医学部細菌学教室

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• コレラ菌由来伝達性Rプラスミッド

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