Candida属菌細胞画分における電子伝達系の分布
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概要
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Subcellular fractions were prepared from cells of Candida albicans and Candida tropicalis by disintegrating them with a French press type apparatus and successive fractional centrifugation. As a result, a cell-wall, a particulate, and a supernatant fraction were obtained. In analyses of these fractions by reducing with dithionite minus oxygenated difference spectra, it was shown that almost all the cytochromes, which were composed of a, b and c, or c plus c1, were distributed in the particulate fraction. Only a small amount of cytochrome was found in the cell-wall fraction and a trace amount of cytochrome c seemed to be distributed in the supernatant fraction. Furthermore, the greater portions of the succiante-and NADH-cytochrome c reductase activities were distributed in the particulate fraction, and small amounts of them in the other fractions. The particulate fraction has already been known to be composed mainly of the intracytoplasmic membrane system.Subcellular fractions of another kind were prepared from sonically disintegrated spheroplast preparations of the cells of both organisms by successive fractional centrifugation. The fractions obtained in this manner were called a mitochondrial, a small particulate, and a soluble fraction. Electron microscopic observation of thin sections of these fractions revealed that the mitochondrial fraction consisted relatively homogeneously of mitochondria, and that the small particulate fraction was composed mainly of small fragments of the outer membrane of mitochondria, vacuoles originated from their inner membrane and other intracytoplasmic membrane systems. In investigation of the distribution of cytochromes in these fractions, it was proved that almost all the cytochrome systems that had been observed mainly in the particulate fraction were now distributed to a similar extent both in the mitochondrial and in the small particulate fractions, and that only a trace amount of cytochrome c was found in the soluble fraction. On the other hand, the succinate-and NADH-cytochrome c reductase activities were the most abundant in the mitochondrial fraction, relatively scanty in the small particulate fraction, and hardly observable in the soluble fraction.
- 日本細菌学会の論文