非病原性ブドウ球菌に対するヒト血清中の抗細菌性物質について (第II報)

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The anti-staphylococcal factor, δ-factor, was taken out, in a partially purified form, from human serum. The properties of the factor and the purified fraction were discussed.The anti-bacterial activity of the factor was lost by dialysis. It was, however, recovered when the dialyzed inside and outside portions had been mixed together. The inactive inside one was reactivated by adding barbital buffer solution.The factor was purified by zone electrophoresis and DEAE cellulose column chromatography.The activity of the purified fraction was 10 times higher than that of the original serum. There was no activity of complement or antibody in the fraction. The active fraction was separated from lysozyme electrophoretically.
日本細菌学会の論文