百日咳免疫の実験的研究 : 第3報攻撃菌のBooster効果について

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The mouse protection test of pertussis antigen by the intracerebral method has been generally accepted as the most satisfactory method of assaying the potency on human bodies. However, the difficulty of this method is the reproducibility, mainly due to the variety of the virulence of the challenge dose.<BR>In the minimum requirement of pertussis vaccine it is stated that the challenge culture should be of such virulence that the LD50 for the virulence control mice contains not more than 500 bacteria according to the plate colony count. From the viewpoint of reproducibility it is considered that this description of the challenge culture might not be enough from the following results.<BR>In this report the results of the protection test by the original 18-323 strain and its substrain are reported. The original strain has been used in Japan without any selection of the colony on the plate for more than 10 years since the introduction from The United States. The LD50 of this strain in this report was 70 organisms by the plate count. The substrain was the strain, which was selected from the original strain on the plate and the LD50 was 10 organisms by the plate count. All of the colonies of this substrain on the plate were haemolytic and smooth.<BR>In the protection test using the original strain the mice challenged by 670 LD50 survived more than the mice challenged by 42 LD50.<BR>On the other hand, in the case of the substrain, there was observed no such result and the mice were killed to more extent with the increased dose of challenge.
日本細菌学会の論文

百日咳免疫の実験的研究 : 第3報攻撃菌のBooster効果について

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