腸内における薬剤耐性腸内細菌について : (1) 健康人の薬剤耐性菌の消長
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Authors made experiments to isolate antibiotic-resistant strains of Enterobacteriaceae from faeces of 68 healthy persons, who were tested once a month from May 1961 to April 1962. Streptomycin (SM), Tetracycline (TC) and Chloramphenicol (CM) were used as antibiotics. The isolation of resistant bacilli was made by plating on MacConkey media and MacConkey media containing 25γ/ml of one of the antibiotics, three or five colonies being fished from each plate. They were tested their drug resistance on heart infusion agar added 25γ/ml and 100γ/m1 of one of three drugs respectively. The results obtained are as follows:<BR>(1) Resistant strains against SM or TC were recovered in markedly greater numbers in summer than in winter, whereas CM resistant bacilli were very few and the seasonal variation of their isolation was, not recognized.<BR>(2) The drug-resistance of 4.745 strains isolated were tested; 1.144 strains (24.1%) were revealed to be resistant to 25γ/m1 and about a half (267 strains) of them resistant to over 100γ/ml of one of three drugs.<BR>(3) During the observation period, drug resistant bacilli were recovered in 63 persons, but constant carriers were very few. It was only in 6 persons that more than 8 times resistant strains were isolated, and no such strains was not recovered in 5 persons.<BR>(4) Only 193 (5.2%) in 3.710 strains recovered on MacConkey agar were drug resistant, these. bacilli were belonging to E. Coli (78.4%), Citrobacter (17.3%), Kledsiella (1.4%) and Proteus (2.9%). The figure of the classification was quite similar to that of the bacilli isolated on MacConkey agar containing drug.<BR>(5) Single-resistant strains were most frequently encountered (73.7%), followed by three antibiotics-resistant strains (16.3%) and two-antibiotics-resistant ones (10.5%). The resistant bacilli against various antibiotics over 100γ/ml were isolated in the following percentage order; SM-single-resistant ones in 4.9%, TC-single-resistant ones in 5.4%, SM, TC-resistant ones in 0.9%, SM, CM-resistant ones in 0.6%, TC, CM-resistant ones in 0.1% and SM, TC, CM-resistant ones in 2.3%. These results were not in accordance with those of dysentery bacilli recovered from patients as well as healthy carriers in these days when these experiments were carried out; about 30% of dysentery bacilli were proved to be antibiotic-resistant, moreover almost all of them were multiple-drugs-resistant.
- 日本細菌学会の論文