Detection of Superoxide Anion Radical in a Stratum Corneum Intercellular Lipid Model Using an Electrochemical Sensor

概要

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A stratum corneum intercellular lipid model was prepared in a quasi-non-aqueous system. It was found that the detection of the superoxide anion radical (O2–•) generated in the lipid model by ultraviolet (UV) irradiation was possible using an electrochemical O2–• sensor. The use of an electron spin resonance–spin trap method confirmed that the reactive oxygen species generated in the lipid model by UV irradiation was O2–•; the presence of a hydroxyl radical (•OH) was also proven. In addition, a reduction in the electric current in the O2–• sensor was observed in lipid models containing added antioxidants such as d-α-tocopherol and β-carotene. Moreover, there was a correlation between the degree of oxidative degradation of the lipid, which was determined by the thiobarbituric acid method, and the electric current due to the O2–• detected using the O2–• sensor.