ASYNCHRONOUS TURNOVER OF THE THIN FILA-MENT PROTEINS, ACTIN, TROPOMYOSIN AND TROPONIN BY A CONTINUOUS DOUBLE ISOTOPE METHOD

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概要

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• The relative turnover rate of protein components of the thin filament was measured by a continuous double isotope method. Both 3H-lysine and 14C-lysine were administered continuously in the diet to male adult rabbits for 7 days. On the 7th day, the 14C-lysine was discontinued while the 3H-lysine was continued for 14 additional days. The relative turnover rate of the protein components was estimated from the 3H/14C ratios of the isolated protein of the same animal. From the different ratios of 3H/14C of each protein fraction, asynchronous turnover of the thin fila-ment proteins (actin, tropomyosin and troponin) was concluded. The order of degree in relative turnover rate is as follows: troponin>tropomyosin>actin.

• 財団法人 学会誌刊行センターの論文

著者

• 船引 龍平

  Muscle Biology Laboratory, University of Wisconsin

• CASSENS R.

  Muscle Biology Laboratory, University of Wisconsin, Madison, Wisconsin 53706, U. S. A.

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• 10.二核種標識継続アイソトープ投与法による繊維構成タンパク-アクチン, トロポミオシン, トロポニン-の非同調性代謝回転(JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY : 掲載論文要旨)
• ASYNCHRONOUS TURNOVER OF THE THIN FILA-MENT PROTEINS, ACTIN, TROPOMYOSIN AND TROPONIN BY A CONTINUOUS DOUBLE ISOTOPE METHOD

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