Effects of Dietary Protein on the Induction of DNA Synthesis and Expression of Growth-Related Genes in Liver and Kidney of Growing Rats.
スポンサーリンク
概要
- 論文の詳細を見る
To investigate molecular mechanisms of growth control by protein nutrition, we examined whether nutritive quality of protein affects the induction of DNA synthesis in liver and kidney of growing rats in relation to expression of growth-related genes such as c-myc, c-fos, c-Ha-ras, and ornithine decarboxylase (ODC). Rats were adapted to 2-h meal feeding schedule at first with laboratory chow for 10 days and then with a protein-free diet for 3 days prior to experiments. When protein-free diet was fed to the rats, the levels of c-myc, ODC and c-Ha-ras mRNAs increased in the liver within 2 days. However, substantial changes in the levels of those mRNAs were not observed in the kidney. The level of c fos mRNA in these tissues was too low to detect by our method. Feeding of casein diet to rats that had been maintained on protein-free diet for 3 days caused a rapid decrease in the level of c-myc mRNA and induced DNA synthesis in the liver. On the other hand, zein diet, which lacks tryptophan and lysine, did not lower the c-myc mRNA level nor induced DNA synthesis in the liver. However, if zein diet was supplemented with tryptophan and lysine, a decrease in c-myc mRNA level and an induction of DNA synthesis were observed. The levels of ODC and c-Ha-ras mRNAs were not changed by feeding of casein or zein diet. Neither casein nor zein induced DNA synthesis and changed the levels of the mRNA in the kidney. The amount of food intake during the 2-h feeding period was not different among the diets. These results suggest that the liver cells are arrested in G 1 phase during the feeding of protein-free diet and good quality of protein is required to progress the cell cycle to enter S phase.
- 財団法人 学会誌刊行センターの論文
著者
-
Hayashi Shin-ichi
Department Of Immunology School Of Life Science Tottori University
-
Hayashi Shin-ichi
Department of Nutrition, The Jikei University School of Medicine
-
KANAMOTO Ryuhei
Laboratory of Food and Nutrition Department of Agricultural Chemistry, Kyoto Prefectural University
-
YOKOTA Tamotsu
Department of Nutrition, The Jikei University School of Medicine
関連論文
- Constant rate of steady-state self-antigen trafficking from skin to regional lymph nodes
- Distinct antigen trafficking from skin in the steady and active states
- Skin antigens in the steady state are trafficked to regional lymph nodes by transforming growth factor-β1-dependent cells
- Estrogen-Related Cancer Microenvironment of Breast Carcinoma
- An Rsa I Polymorphism in the CYP2E1 Gene Does Not Affect Lung Cancer Risk in a Japanese Population
- Loss of estrogen receptor β isoform expression and its correlation with aberrant DNA methylation of the 5'-untranslated region in human epithelial ovarian carcinoma
- Midkine and its clinical significance in endometrial carcinoma
- Expression of retinoic acid receptors in human endometrial carcinoma
- ABNORMAL POLYAMINE METABOLISM IN HYPERTENSIVE CARDIAC HYPERTROPHY
- Cloning and Sequencing of a Gene Encoding D-Aminoacylase from Alcaligenes xylosoxydans subsp. xylosoxydans A-6 and Expression of the Gene in Escherichia coli
- CIRCADIAN FLUCTUATION IN CARDIAC POLYAMINE CONTENTS OF THE DIABETIC RATS : Cardiac Metabolism : FREE COMMUNICATIONS (II) : PROCEEDINGS OF THE 49th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
- Estrogen signaling pathway and hormonal therapy
- Contribution of Neural Crest Cells in Tooth Development and the Possibility of Tooth Regeneration
- Basic Research for Hormone-Sensitivity of Breast Cancer
- Reduced Size of Preputial Glands and Absence of Aggressive Behavior in the Genetically Obese (ob/ob) Mouse : COMMUNICATION : Behavior Biology
- Presence and distribution of neural crest-derived cells in the murine developing thymus and their potential for differentiation
- Estrogen signaling pathway and its imaging in human breast cancer
- Mutational Analysis of Uncharged Polar Residues and Proline in the Distal One-Third (Thr^-Pro^) of the Highly Conserved Region of Mouse Slc10a2
- Soybean Resistant Protein Elevates Fecal Excretion of Cholesterol and Bile Acids and Decreases Hepatic Cholesterol Content in Comparison with Soybean Protein Isolate
- Effects of Ala Substitution for Conserved Cys Residues in Mouse Ileal and Hepatic Na^+-Dependent Bile Acid Transporters
- Taurocholic Acid Does Not Induce Apoptosis in HCT116 Cells Regardless of Its Intracellular Concentration
- Characteristics of Apoptosis in HCT116 Colon Cancer Cells Induced by Deoxycholic Acid
- Characterization of Cloned Mouse Na^+/taurocholate Cotransporting Polypeptide by Transient Expression in COS-7 Cells(Biochemistry & Molecular Biology)
- Effects of Cys Mutation on Taurocholic Acid Transport by Mouse Ileal and Hepatic Sodium-dependent Bile Acid Transporters(Biochemistry & Molecular Biology)
- Antitumorigenic Effects of Several Food Proteins in a Rat Model with Colon Cancer and Their Reverse Correlation with Plasma Bile Acid Concentration
- Characterization, cDNA Cloning, and Fuctional Expression of Mouse Ileal Sodium-Dependent Bile Acid Transporter
- Endemic Goiter in Sarawak, Borneo Island; Prevalence and Pathogenesis
- Sex steroid hormones in pairs of tumor and serum from breast cancer patients and pathobiological role of androstene-3β, 17β-diol
- Effects of Dietary Protein on the Induction of DNA Synthesis and Expression of Growth-Related Genes in Liver and Kidney of Growing Rats.
- c-myc mRNA is Stabilized by Deprivation of Amino Acids in Primary Cultured Rat Hepatocytes.
- Is Preoperative Ultrasound Examination Useful for Detecting Contralateral Patent Processus Vaginalis in the Groin Hernias of Children?