Induction of human peripheral blood lymphocyte activation by supernatant of sonicated Actinomyces viscosus.
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The supernatant of sonicated Actinomyces viscosus (AVsup) and Actinomyces Israelii (Alsup) were examined for their mitogenecity to human peripheral blood lymphocytes.<BR>Peripheral blood mononuclear cells were separated into three groups: 1) mononuclear cell (unseparated) fraction, 2) T cell enrich (E-receptor positive cell) fraction, 3) B cell enrich (E-receptor negative cell) fraction.Each cell fraction was cultured with AI and AVsup respectively for 72 hrs and DNA synthesis was assayed. Streptococcal preparation (OK-432) and PWM were also tested as positive controls.<BR>AVsup induced a low increase of DNA synthesis on T cell enrich and B cell enrich fraction, but a higher increase on mononuclear cell fraction. Alsup did not induce any increase of DNA synthesis. OK-432 and PWM induced higher DNA synthesis on T cell enrich fraction as compared with B cell enrich fraction, and on mononuclear cell fraction, DNA synthesis was induced higher than T cell enrich or B cell enrich fraction. Furthermore, the change of T cell subsets were observed using FITC conjugated monoclonal antibodies at 24, 48, and 72 hrs cultures.AVsup did not induced increase of OKT3 (T cell), OKT4 (helper/inducer T cell), OKT8 (suppressor/cytotoxic T cell) antigen positive cell population.However, it increased OKDR antigen positive cell population included activated T cell, 70% of B cells, 55% of monocytes, but only monocytes were excluded by appropriate gating on the cytogram of the flow cytometer, although it failed to increase OKB7 (95% of B cells) antigen positive cell population.So the increasing of OK DR antigen positive cell population was considered not as B cells but activated T cells.<BR>It might be strongly suggested that AVsup induced T cell activation without being accompanied with remarkable blastogenesis and proliferation, and then the B cell was stimulated by the activated T cell.
- 社団法人 日本口腔外科学会の論文
社団法人 日本口腔外科学会 | 論文
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