フィブロネクチンを産生するヒト舌扁平上皮癌由来細胞株の樹立とその性状

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A fibronectin-producing cell line, called NA cell, has been established from explant culture of a primary squamous cell carcinoma of the tongue. Continuously propagated over 400 passages, NA cell shows a polygonal shape and monolayer growth. Morphological examination revealed the cell as having epithelial and malignant characteristics. The population doubling time of NA cell is approximately 21. 6 hours. The cell cycle was analyzed by means of a pulse-labeling-chasing method, showing a 15-hour cell generation time consisting of 6. 3 hours G<SUB>1</SUB> period, 4. 5 hours S period and 4. 2 hours G<SUB>2</SUB> period. The cells were implanted in BALB/C-nu/nu nude mice and histopathological findings of the implanted tumor resembled the original structure. Ultrastructural observation of the implanted tumor revealed presence of desmosornal attachments between cells and cytoplasmic tonofilaments. In karyotype analysis, the patterns of chromosomal distribution were mostly hypertriploid, mode 76.<BR>The location of fibronectin was investigated with an avidin-biotin complex immunoperoxidase technique (ABC), revealing fibronectin distributed on the cell surface and in the cytoplasm around the nuclei. The synthesis of fibronectin was then examined by an enzyme-linked immunosorbent assay (ELISA), revealing an increase of fibronectin in the conditioned media in a time-dependent manner until the cell reached the stationary phase.<BR>As these data indicate that this cell line produces fibronectin, NA cells may be useful for studying mechanisms of metastasis and invasion of tongue cancer.
社団法人日本口腔外科学会の論文

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