口腔癌細胞核DNA量に関する研究 : 第1報各種試料処理方法間の検討

概要

論文の詳細を見る
Recently, there are variousmethods of nuclear DNA contentmeasurement andmaterial treatment. So we comparatively studied variousmethods ofmaterial treatments for nuclear DNAmicrophotometry usingmMSP-TU system (OLYMPUS).<BR>Objects consisted of 10materials from 9 cases which were all primary squamous cell carcinoma of the oral region. Wemeasured nuclear DNA content from specimens of 5μm and 10μm thick, and cell isolation of 30μm thick paraffin. And according to t e classification of nuclear DNA pattern, we considered an error which exsisted between threematerial treatments.<BR>Results: Identical rate of nuclear DNA pattern between groups of cell isolation of 30μm paraffin thick and specimen of 10μm thick showed 90%, which were higher compared to identical rate (60%) of nuclear DNA pattern between groups of cell isolation of 30μm thick paraffin and specimen of 5μm thick.<BR>We think themeasurement of nuclear DNA content from the 10μm thick specimen is easy and valuable.