骨形成タンパク (BMP) の賦形材の検討

概要

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Hydroxyapatite cement (HAP cement) and fibrin glue were evaluated as a delivery system of bone morphogenetic protein (BMP) in terms of roentogenographic and histological method.Semipurrified BMP was prepared from bovine bones according to Urists method, and the BMP showing bioassay was provided as a material. 5 mg of BMP mixed with 20 mg of HAP cement or 0.2 ml of fibrin glue was implanted into ICR mouse thigh muscle, and post-survival rates were 3, 5, 7, 14, 21 and 28 days. Chondroid matrix existed in the HAP-BMP implanted area on day 14 post operation, osteoid tissue on day 21, and endochondral ossification on day 28.In the newly formed chondro-osseous tissue, the particles of HAP cement still remained.On the contrary, mixture of BMP-fibrin glue resulted in the formation of connective tissue without finding any evidence of bone tissue formation. From the results of the present study, the author evaluated that HAP cement is a useful material as a BMP delivery system.