Trypticase Soy Agarを用いたCup Plate法による唾液過酸化酵素抗菌活性の検定

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The peroxidase antibacterial action in human saliva against species of lactobacilli has been described by Klebanoff and Luebke, Iwamoto et al. Inoue, Saji, Morioka and Matsumura have reported that the antibacterial activity is distributed in certain tissues of rat including parotid, submaxillary, lacrimal, and mammary gland. In each study, however, this antibacterial activity was detected in SL broth against lactobacilli. Inoue et al. have also reported that the salivary antibacterial (S.A. Factor) activity was obtained in the selected media including Trypticase soy broth by treating with the-SH group reagent, CuSO4. It is conceivable that the salivary peroxidase antibacterial system plays an important role as a natural defensive function in the oral cavity. In order to clarify the defensive function of the antibacterial system, nonselected culture medium such as Trypticase soy other than SL broth should be used obtaining the wide variety of antibacterial spectrum against oral microorganisms. As the growth rate of the oral microorganisms in culture media, in general, is low, an estimation of the growth inhibition by the antibacterial system on the microorganisms is hard to quantify, and the viable cell count of the certain microorganisms such as cariogenic streptococci is also hard to carry out. It is suggested that in these cases the antibacterial activity would be adequately estimated by the cup plate method. In the present work, experiments were carried out to determine whether the antibacterial activity could demonstrate quantitatively in the non-selective medium such as Trypticase soy. The salivary peroxidase antibacterial activity was successfully demonstrated in the cup plate method with Trypticase soy agar containing 8.3×10-5M CuSO4. Growth of Lactobacillus plantarum ATCC 8014 was apparently inhibited by the antibacterial system, and the growth inhibition is completely depressed by catalase. The cup plate method is strongly suggested to be useful for the quantitative estimation of the salivary peroxidase antibacaterial activity against various species of oral microorganisms.
有限責任中間法人日本口腔衛生学会の論文

Trypticase Soy Agarを用いたCup Plate法による唾液過酸化酵素抗菌活性の検定

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