乳酸菌溶解酵素およびテンペレートファージの産生条件について

概要

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The lytic enzymes acting on lactobacilli were found in the phage lysates induced from lysogenic bacteria, group C L.casei strains IAM 1043, R-094, C-47, C-201, and 3-793, out of 36 strains tested. In the process of induction of phage development in lysogenic strain IAM 1043, the lysis was affected by the growth temperature and pH value of the culture medium in the presence of 0.3μg/ml of mitomycin C even at the early log phase culture. Both phage-like particles and lytic enzymes were first detected intracellurary at 180 minutes after addition of mitomycin C to the culture medium. The enzymes were not detectable in the culture of lysogenic strain without inducing treatment. The hypothesis that the lytic enzymes synthesis was directed by the phage genome was discussed.
社団法人日本農芸化学会の論文