Leuconostoc mesenteroidesによる5'-D-riboflavin-α-D-glucopyranosideの生成機作-1-

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Experiments to elucidate the mechanism of 5'-D-riboflavin-α-D-glucopyranoside (B2-5'-α-gl) formation by Leuconostoc mesenteroides were carried out with enzyme preparations from two strains: NRRL B-512 having lower B2-5'-α-gl-forming and higher dextran-forming activities, and NRRL B-1299 (=IAM 1151) having higher B2-5'-α-gl-forming and lower dextran-forming activities. Rates of glucoside formation of isoalloxazine derivatives from sucrose and D-araboflavin (or D-galactoflavin) by enzyme preparations from two strains were lower, as compared with rates of B2-5'-α-gl formation. Ribitol was the best alternate glucosyl acceptor among various sugar alcohols tested, when present during dextran formation by the enzyme preparation from the strain B-1299. Ribitol sharply increased the rate of production of reducing power while the yield of native dextran was decreased. Moreover, ribitol glucoside like substance was detected on a paper chromatogram. While, in dextran formation by the enzyme preparation from the strain B-512, ribitol also acted as glucosyl acceptor, but its ability was much less efficient. From these results, it was considered that the yield of B2-5'-α-gl by enzyme preparations from two strains of Leuc. mesenteroides was closely related with their strength of acceptor affinity to the ribitol in B2 molecule for glucosyl group.
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