各種レブリン酸資化性細菌によるレブリン酸の資化 (微生物によるレブリン酸の代謝-2,3-)

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Assimilation of levulinic acid by three strains of levulinic acid-utilizing bacteria, Brevibacterium linens D-87-11-2 (Strain B), Corynebacterium equi 13-9 (Strain C) and Pseudomonas aeruginosa M-101-1-1 (Strain P) were studied. These strains assimilated levulinic acid in simple media containing nitrogen source, potassium phosphate, magnesium sulphate and levulinic acid, Addition of organic nutrients such as yeast extract, corn steep liquor and meat extract stimulated the assimilation of levulinic acid. B. linens D-87-11-2 required biotin for growth in the media containing levulinic acid or glucose as a sole source of carbon. B. linens D-87-11-2 and P. aeruginosa M-101-1-1 could assimilate levulinic acid in the media containing 5% or more of levulinic acid but C. equi 13-9 could not assimilate it if levulinic acid in the medium became to 3% or more. In the media containing both levulinic acid and glucose, glucose assimilation followed after levulinic acid assimilation by B. linens D-87-11-2 and C. equi 13-9 but glucose was assimilated preferentially by P. aeruginosa M-101-1-1. Neither organic acid except levulinic acid nor ketone was found in cultured broths of these bacteria.
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