Saccharomyces cerevisiaeとMicrococcus sp.の混合培養に関する研究-3-酵母のミオイノシトール分泌について(ノート)
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When Saccharomyces cerevisiae was cultured with shaking in a glucose-ammonium sulfate medium free from myoinositol, cells excreted a considerable amount of myoinositol to the medium at the late stationary phase of growth. Under optimal conditions, the yield of myo-inositol, that was excreted, exceeded 30μg/ml of the culture medium. When the cells were grown in the medium containing a small amount of added myoinositol (1μg/ml), growth was slightly stimulated but the excretion of myo-inositol decreased considerably as compared to the culture without myo-inositol. Time course study of myo-inositol formation by the yeast cells revealed that myo-inositol was synthesized concomitant with growth and existed predominantly as bound forms in the early stationary phase cells and that it was released thereafter from the cells as free and bound forms upon a further incubation, since no significant change in the amount of total myoinositol was detectable throughout this period. Present address: Laboratory of Food and Nutrition, Fukushima Women's Junior College, Fukushima
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