ウリカーゼに関する研究-2-放線菌ウリカーゼの精製ならびに精製酵素の性質
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概要
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Uricase was extracted by ultrasonic oscillation from cells of Streptomyces sp. grown on a medium containing urate. The enzyme was purified about 600-times by ammonium sulfate fractionation, acetone fractionation, DEAE-Sephadex column chromatography and Sephadex G-200 gel filtration. The purified enzyme had an optimum pH at 8.0 and optimum temperature between 30° to 50°C for the reaction in borate buffer, and was stable at pH higher than 6.0 and at temperatures lower than 50°C. Streptomyces uricase, as well as animal one, exhibited a substrate inhibition and was competitively inhibited by xanthine. Km for urate and Ki for xanthine of the purified enzyme in borate buffer (pH 8.0) were 2.5×10-5M and 1.3×10-4M, respectively.
- 社団法人 日本農芸化学会の論文