クコのビタミンB1不顕化物質に関する研究-1-

概要

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An investigation has been made here into the masking effect of an extract of Lycium chinense-the so-called "Kuko"-upon the qualitative thiamine reaction. And the fact, that the factor in the extract responsible for thiamine-decomposition is not attributable to an enzyme, has been proved by the following: (a) The factor was thermostable, for, even when the extract was boiled about an hour, its thiamine-decomposing ability was not decreased. (b) Thiamine-decomposition was completed in a moment and did not increase as time passed on. When cysteine or ascorbic acid was added to a thiamine solution, the diazo reaction of which was masked by adding the extract to it, the reaction was normally regained. The mother substance which has relations with the masking effect was adsorbed into the lead subacetate, anhydrous. When one-dimensional ascending paper chromatography was carried out by using n-butanol-acetic acid and water (2:1:1), Rf value of the substance showed 0.55. Being a substance of yellowish brown-needle-like crystals while soaked in dehydrated ethanol, it turned completely into a brownish resinoid substance the moment ethanol was removed. One mg of the substance could mask 3μg or more of thiamine under the experimental condition given by the author.