糸状菌のアミラーゼに関する研究-1-γ-アミラーゼの精製
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概要
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Three different types of amylase, glucoamylase, γ-amylase and α-amylase, were isolated and purified from a wheat bran culture filtrate of Aspr niger strain gamma by chromatographies on SE-sephadex column. The specific activity of γ-amylase was increased to about 100 folds comparing with that of the original solution. The reaction products from various substrates by γ-amylase are as follows: Polysaccharides; Soluble starch, amylopectin, amylose, glycogen gave only glucose as reducing sugar. Disaccharides; Maltose and isomaltose could not be attacked at all. Oligosaccharides; Maltotriose, isomaltosyl-maltose and phenyl maltoside are more or less attacked by γ-amylase and glucose was always detected.