Glutathioneに關する研究 (第5報) : Glutathioneの定量法中沃度法.沃度法中小川法藤田・沼田法の選擇及び結晶純度に就て
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The methods for determination of glutathione (GSH) ever published were classified according to my own view as follows: Iodometric method (I), 2) Colorimetric method (II ), 3) Cadmium method, 4) Glyoxalase method, and 5) the others. (I) had been particularly researched and revised by many investigators, but it is not, yet settled, because various known or unknown SH contg. substances besides, GSH are present in living organisms and the other substances which consume iodine or interfere SH-determination reagents may be present. Problems in (I) are summarized as follows: 1) Indicator problems (external indicator or internal ind icator), 2) Discrepancy problems between (I) and (g ), 3) Propriety problems between direct and indirect titration, 4) Problems concerning reduction of GSSG, 5) Problems concerning oxidation of GSH, and 6) Problems concerning removement of materials which consume iodine without effecting on GSH. The finding of 4 factors for (I) made by OKUDA and OGAWA seemed to settle the problem. But, in 1938, OGAWA and FUJITA & NUMATA presented res . his and their own (I), which, since then, represented two different standpoints. It is the reason why I selected these two methods among them when I tried to reexamine the above problems in (I). I must show the purity of my GSH crystal which was used in my all examinations before I state my results. It is difficult to decide the purity of GSH, crystal by only one test, because the theoretical values of elementary analysis for its two types bear a close resemblance and also, its mp. indicates simultaneously its decomposition point in the case of peptide as GSH, therefore its mp. is everchanging according to heating conditions. I decided the purity of my crystal by testing 7 items shown in Table 1.
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関連論文
- Glutathioneに關する研究 : (第6報)Glutathioneの定量法中沃度法・Glutathioneの沃度定量に就て
- Glutathioneに關する研究 (第5報) : Glutathioneの定量法中沃度法.沃度法中小川法藤田・沼田法の選擇及び結晶純度に就て