醗酵による纖維の精練に就て : (第7報)黒黴の液内培養酵素液による苧麻纖維の精練に就て

概要

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We investigated the microbiological degumming of ramie-fiber by using a strain of Aspergillus nicer isolated from raw ramie. The submerged culture-fluid was used for that purpose so that the retting-time may be saved and the back spores may not damage the fiber. In the shaking culture, production of depectinizing enzyme is accelerated by use of alkali scouring waste liquor of ramie as culture medium. The addition of stillage as nutrient is highly effective. The less the amount of C source was added to alkali scouring waste liquor (the basal medium), the more the amount of protopectinase was produced, so far as the develop-ment of the mold and the production of oxalic acid were concerened. The highest activity of protopectinase out of influence of oxalic acid was obtained on the following cultural conditions: media: A. S. W. L, 100+glucose 2+peptone 1; initial pH: 5.2_??_7.6; temp.: 29_??_35°C. Though conditions of retting are not yet fully studied, we can expect cooperative effect of the enzyme and oxalate produced in the culture, so that the fiber may be degummed in short-er time than a day at the temperature as low a_??_30_??_40°C.
社団法人日本農芸化学会の論文