細菌胞子の耐熱性に関する研究(1)-耐熱性測定方法並びに数種の因子の影響に就て-

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(1) The method we used to determine the heat resistance of spores of aerobic bacteria is a modification of the method of O. B. WILLIAMS (1929). The spores were harvested from nutrient agar slant, suspended in sterile M/15 phosphate buffer of pH 7.0, and filtered through a sterile filter paper to remove clumps of spores. The spore suspension was counted by means of haernacytometer (Thoma) and diluted so as to contain a definite number of spores. Then the suspension was pipetted into small glass tubes and heated in a boiling water bath. (2) The "basic heat resistance" of spores of the aerobes (i.e. the survival time at 100C. with spore concentration of 50 millions per ml.) are as follows; Bac. subtilis…………10 minutes, Bac. mesentericus………10 minutes, Bac, megatherium…8 minutes, Bac. mycoides…………10 minutes Bac. natto……………16 minutes, (3) The unfiltered spore suspension containing spore clumps shows higher heat resistance than the filtered suspension. But in the former case the "skips" occurs and it is impossible to obtain definite results. (4) The effects of several factors on heat resistance of spores of Bac. subtilis were pursued. (The results are shown in Tables 3, 4, 5 and 6)
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