細菌マンナン分解酵素による蒟蒻糊抜に就て

概要

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We found a useful bacterium for desizing of ramie cloth sized with konjac (Amorphophallue kenjac). This bacterium secreted powerful mannanase which degraded konjac mannan into a lower polymer, which lost coagulability with alkali. For preparation of the desizing agent, solid culture method is costless and suitable. The suitable medium, such as neutralized rice bran with the same volume of water, is cooked and inoculated. Within 2 or 3 days-cultivation at 30-37°C, the enzyme activity rises up to the maximum ; this may be .dried in the air. The mannanase is produced. enough even in the absence of the appropriate substrate, konjac mannan. Optimal conditions for the enzyme action are at pH 6 and 50°_??_60°C; below pH 5 and over 70°C the mannanase is considerably inactivated, but its high activity in a relatively wide range of pH 6_??_8 andin higher temperatures makes it utilizable for industrial purposes. The desizing is desirable to be carried on at a relatively lower temperature and with a higher concentration of the agent; reuse of the residual liquor is possible.
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