稻馬鹿苗病菌の生化学 : (第31報)ヂベレルンの物理化学的定量法

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The bioassay using rice seed was the only method för the quantitative -determination of gib-berellins. But this method is not so good, because not only it takes about 10 days, but also the result is unreliable on account of the individuality of every seed. Therefore, it is hoped that some physico-chemical methods should be found as soon as possible. For this reason, this experiment was undertaken and the following results. were obtained. The authors consider the polarographic method under described is very useful forte studies of gibberellins, such as the studies on the medium composition, preparing process and mode of action in living cell. 1) We found that the relation between the extinction coefficient and the concentration of gibberic acid was proportional satisfying LAMEERT-BEERs law, and therefore gibberic acid could be determined quantitatively by the spectrophotometric measurement. (Fig. 1) 2) We found that gibberellin A gave a reduction wave In a 0.5×10-3, M solution of this substance, when 0.04 N, tetra-methylammonium bromide being used as a supporting electrolyte, the half-wave potential was -1.56 volt (vs. S. C. E.) as shown in Fig. 2. As the relation of the wave-heights of gibberellin A to its concentration was found to be proportional linearly, gibberellin A could be determined quatitatively by means of the polarographic method. (Fig. 3) 3) Except gibberellin A and gibberic acid, the acids such as gibberellin C, fusaric, succinic, fumaric and oxalic acids gave the other reduction potentials. From this fact it was found that gibberellin A in the medium could be determined quantitatively and, moreover, its accuracy was indorsed from the result of bioassay which was carried out as the comparison, at the same time.
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稻馬鹿苗病菌の生化学 : (第31報)ヂベレルンの物理化学的定量法

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