カトレヤの茎頂培養による栄養繁殖法に関する研究-1-茎頂組織の活着と生育に影響を及ぼす要因について
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The unstability of growth initiation from bud-meristem explants of Cattleya alliance in vitro is considered to be a great problem to establish a technique of clonal propagation by tissue culture method. So some factors which affect survival or growth of the explant were studied. The meristems were excised from apical or lateral buds of new shoots and cultured on RM-1962 medium under continuous llumination of fluorescent lamps about 2000lux and 25°C.1. NAA or 2, 4-D is essential for the growth initiation of explants and the optimum concentration is found each at 0.1ppm.2. The culture medium of RM-1962 in liquid state is better both for the survival rate and growth amount of explants than that of Knudson C. The organic components of RM-1962 had little effects on the growth and so the mineral part of the medium is supposed to be effective. The effect of addition of coconut milk at 10% to the medium differed among the hybrids used.3. When the explants were cultured in liquid standing, liquid rotating at 0.86 rpm, liquid shaking at 90rpm and on agar-solid media, the former two were confirmed to have a good effect for survival and suppression of tissue-browning and the latter two to promote tissue-browning and then to decrease the survival rate of the explants.4. The explants from apical bud and lateral buds on upper nodes of a shoot tended to be inferior in the survival and growth rate to the explants from middle and lower nodes.5. The growth response of meristems seemed to differ by each species and hybrid used even if cultured under the same condition.
- 園藝學會の論文
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- カトレヤの茎頂培養による栄養繁殖法に関する研究-1-茎頂組織の活着と生育に影響を及ぼす要因について