Applications of quantitative endotoxin assay using fluorescent probe method. Special reference to endotoxin clearance.:Special Reference to Endotoxin Clearance

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Endotoxin concentration in city water increased following the storage at room temperature and decreased by applying purifications-systems determined using the fluorescent probe method.High concentrations of endotoxin were detected in pleural and peritoneal fluids, bile and urine with positive cultures of gram-negative rods. Plasma endotoxin concentrations in rabbits were decreasing exponentially after administration of endotoxin, and then the plasma disappearance rate (K) was able to be calculated. The maximum removal rate (Rmax) was also able to be calculated, because K values decreased proportionally to increased administered endotoxin doses. K and Rmax apparently decreased when rabbits were pretreated with dextran sulfate, and K elevated and Rmax were similar to the controls when the animals were pretreated with zymosan. These results suggested that at least two disposing mechanisms are involved in the clearance of endotoxin by the reticuloendothelial system.