Fetal calf serum-augmented cytotoxicity of peripheral blood mononuclear cells obtained from patients with cancer of the digestive organs.
スポンサーリンク
概要
- 論文の詳細を見る
The cytotoxic activity of peripheral blood mononuclear cells (MC) as been reported to be enhanced after they were cultured in media containing fetal calf serum (FCS). This study was designed to investigate the cytotoxic activity of MC obtained from patients with cancer of the digestive organs before and after culture in FCS-supplemented medium. The cytotoxicity of MC obtained from healthy subjects was likewise investigated for reference. Raji cells and K-562 cells were used as target cell lines. The cytotoxicity of FCS-cultured MC to Raji cells and K-562 cells rose in healthy subjects to the levels 10.0± 10.4 times and 2.2±1.4 times those before culture, respectively. In patients with gastric cancer, however, this variable rose only to the levels 3.5±5.3 times and 1.2±1.3 times those before culture, respectively. The percent increase in the cytotoxicity of MC to Raji cells and K-562 cells after culture became lower in patients with gastric cancer with advance in stage and also in patients with the cancer of other digestive organs mainly at stage IV. No relation was noted between the cytotoxicity and blastogenesis of FCS-cultured MC. Changes in lymphocyte subpopulations after culture were also investigated using OKT4, OKT8 and Leu7 in patients with cancer of the digestive organs. Both OKT4 and OKT8 subpopulations increased, but the ratio of these subpopulations remained almost unchanged. Leu7 subpopulation significantly increased (p<0.05). The above findings suggest that the cytotoxic effectors generated in this study by culturing human MC in FCS-supplemented medium resemble NK cells, and that the FCS-augmented cytotoxicity of MC may possibly serve as a parameter of cellular immunity in patients with cancer of the digestive organs.
- 財団法人 日本消化器病学会の論文
著者
関連論文
- 拡張型心筋症患者の胆石治療手技経皮経肝胆嚢鏡下截石術を用いて
- トルネードコイルを用いた持続動注カテーテルの1ルートによる固定法
- 胃支配動脈と肝細胞癌に対する経カテーテル的治療後の急性胃・十二指腸病変との関係 : 特に右胃動脈について
- レーザ分光法を用いた同位体分析計と ^C-呼気検査応用
- 早期胃癌の深達度診断における内視鏡的検討
- ^C-尿素呼気試験における口腔内での^CO_2ガス発生とその影響
- ^C-acetate呼気試験による胃排出能の評価
- プロトンポンプインヒビターによる胃粘膜の炎症細胞浸潤の減少と Helicobacter pylori の消失について
- MALLORY-WEISS 症候群198例の検討
- Helicobacter pylori 診断における安定同位体呼気検査の検討
- 標識法の違いによるヨード標識モノクローナル抗体の細胞での代謝の差異について
- 本邦で最初に確認したマンソン住血吸虫症の1例
- 急性膵炎によりもたらされたケトアシドーシス性糖尿病性昏睡の1例
- 壊疽性膿皮症を合併した潰瘍性大腸炎の1例
- SIADHを呈した食道小細胞性未分化癌の1例
- WDHA症候群を呈した多種ホルモン産生膵腫瘍の1例
- Optimal branching of hepatic artery in patients with liver cirrhosis.
- A case of eosinophilic peritonitis with giardiasis.
- A case of mucinous cystadenoma in the tail of the pancreas accompanied with tubular adenocarcinoma in the head.
- Fetal calf serum-augmented cytotoxicity of peripheral blood mononuclear cells obtained from patients with cancer of the digestive organs.