Mycobacteria in Crohn's disease.

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概要

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• Recently Chiodini, et al. described isolation of Mycobacterium species from several patients with Crohn's disease (CD). These data prompted us to investigate the relationship between M. Paratuberculosis and CD. Using ELISA, no difference was observed between CD and ulcerative colitis (UC) in antibody titers against M. paratuberculosis in any classes of immunoglobulin, although the titers of CD were slightly higher than those of controls in IgG and IgA classes (P<0.05). Using Herrold's egg yolk medium (HEYM) with mycobactin, we attempted to isolate organisms from tissue specimens and stools. After three weeks of culture, whity spots were seen on the HEYM in which the sample of the biopsy specimen from a patient with CD was added. These isolates were coccoid and positively stained with Ziehl-Neelsen method showing dissimilarity to the Mycobacteria isolated by Chiodini, although subculture was not successful. Using PAP method with antibody against M. paratauberculosis, we attempted to stain intestinal specimens from patients with CD, resulting in no positive stain in any specimen. In conclusion, we could not get any evidence to support that M. paratuberculosis plays an etiological role in CD.

• 財団法人 日本消化器病学会の論文

著者

• 芳賀 陽一

  弘前大学医学部第一内科

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• Mycobacteria in Crohn's disease.

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