Experimental studies on mechanism of gastric acid secretion. Ultrastructural changes of parietal cells in the isolated gastric glands from guinea pigs stimulated with histamine.:Ultrastructural Changes of Parietal Cells in the Isolated Gastric Glands from

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Ultrastructural changes of the parietal cells and changes of intracellular cyclic AMP with histamine stimulation were sequentially observed in the isolated guinea pig gastric glands. Cells were classified morphologically into four groups: "Resting": tubulovesicles were dominant. "Stimulated": secretory canaliculi were expanded. "Partially stinulated": intermediate between former two groups. "Other": cells with collapsed secretory lumina or dense membranous profiles. And the proportions of numbers of these classified cells under each condition were investigated. At 10 minutes after stimulation with 10-3M histamine the proportion of "stimulated" increased, at 20 minutes "other" increased and at 40 minutes proportions returned to the same value as before stimulation. "Other" were considered to be the cells which were returning from "stimulated" to "resting" morphologically. The increase of "stimulated" at 10 minutes was in a dose-responsive manner and the addition of cimetidine blocked this increase. The proportion of "stimulated" paralleled to the intracellular cyclic AMP and a direct relationship between the activity of cyclic AMP and morphological changes in the parietal cell stimulated with histamine was suggested.
財団法人日本消化器病学会の論文