Prostaglandins in rat gastric mucosa. 11th Report. Effect of hypoxia.:Effect of Hypoxia

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We investigated the effect of gastric hypoxia on the endogenous prostaglandins (PGs) in the gastric mucosa in vivo and in vitro experiments using rats.In vivo study, rats with gastric fistulation were instilled saline every 30min, and PGs released into gastric lumen were determined by radioimmunoassay. In control rats, releases of PGE2 and PGI2 were excessively high in the early experimental period followed by the gradual decrease, and then stable releases were obtained after 60min. Therefore, all the in vivo experiments were performed after 60min-resting time. By the ligation of left gastric artery, release of PGE2 was markedly increased. In vitro experiments, PGE2 and PGI2 released from rat isolated gastric wall were significantly decreased by bubbling with anoxic gas (N295%, CO25%) compaired with O2 saturated controls (O295%, CO25%).These results suggest that gastric hypoxia in vivo indirectly stimulates the synthesis of PGs in gastric mucosa. However, the mechanisms are still unelucidated.