Postsecretory changes of pancreatic juice trypsinogen in the duodenum. II. Trypsin to amylase ratio in duodenal aspirates indicates the degree of activation of trypsinogen.:II. Trypsin to Amylase Ratio in Duodenal Aspirates Indicates the Degree of Activat

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During incubation of rabbit pancreatic juice, duodenal juice and bile at 37°C, amylase activity was constant, while amount of activated trypsin varied widely in dependance on bile content. The activity ratio of trypsin to amylase (T/A ratio) in duodenal aspirates, therefore, indicated the degree of activation of trypsinogen into trypsin. In a case of posttraumatic pancreatic fistula, T/A ratio in the duodenal aspirates varied from 5 to 50% of the constant ratio of trypsinogen to amylase in the pancreatic fistular secretions. T/A ratio in the duodenal aspirates of normal subjects increased with increase of bile content which was proportional to calcium concentration, reaching a plateau at the icteric index of 150 or the calcium concentration of 1.2mM. In cases of disturbed bile excretion into the duodenum, a remarkable decrease of trypsin output in the duodenal aspirates was found after stimulation of CCK-PZ and secretin inspite of no significant change in amylase output. It is concluded that human pancreatic juice trypsinogen, immediately after being secreted into duodenum, is activated partially as a result of concurrent degradation, while amylase recieves no change. The degree of activation of trypsinogen is indicated by T/A ratio in duodenal aspirates and estimated to be about 5 to 50%, depending on bile content.
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