A Study of the Mechanism of Antipeptic Substances:(1) Inhibition of peptic proteolysis by polysulfated glycopeptide of hog intestinal origin

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Studies were pursued on the mechanism of an inhibitory effect of polysulfated glycopeptide (GLPS) extracted from hog intestinal mucosa on the proteolysis of albumin substrate.In agar gel plate, precipitation reaction was observed between GLPS and albumin substrate, but there was no precipitation reaction among pepsin, N-acetyl-phenylalanyl-diiodotyrosine and GLPS.In condition of a constant quantity of albumin substrate and of GLPS, variation of pepsin concentration did not affect the proteolytic inhibition rate of GLPS.In certain range of GLPS concentration, proteolytic inhibition was dependent on albumin concentration, however, the quantity of substrate protected by a given amount of GLPS was independent of the concentration of the substrate and of GLPS.It was assumed that the mechanism of the inhibition of peptic proteolysis by GLPS involved primarily the formation of a complex between albumin substrate and GLPS.However, the kinetic analysis showed that a complex formation between substrate and GLPS would not be a sole explanation for the mechanism and more intricate mechanism seemed to be involed in the proteolytic inhibition by GLPS.
財団法人日本消化器病学会の論文