Prolactin Bioassay : Its Sensitivity and Stability
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概要
- 論文の詳細を見る
During past several decades, a variety of bioassay procedures for prolactin has been developed, but preference between each procedure was not yet precisely demonstrated. Therefore following experiments were carried out by using rabbits, mice, rats and pigeons.<BR><I>In vivo</I> mammary gland assays, such as systemic and intraductal injection into pseudopregnant rabbits, were revealed insensitive, variable and laborious. Minimum effective doses were 1 IU of prolactin in systemic injection and 0.5 IU in intraductal injection. But there were no different findings between 1 and 8 IU in systemic, and 0.5 and 3 IU in intraductal injections.<BR>Prolactin has a luteotropic action in some animals. Corpus luteum cell nuclei (CLCN) of immature hypophysectomized rats were counted after PMS, HCG and prolactin were treated for 10 days. Prolactin stimulate growth of CLCN and therefore numbers of CLCN decreased per unit area. Prolongation of diestrus and positive reaction of deciduomata were observed in mice following prolactin injection, but they were not so sensitive and stable as pigeon assay.<BR>β-glucuronidase activity of rat testis were also revealed to be not sufficiently enough to detect small amounts of prolactin.<BR>Pigeon crop-sac assays are available for prolactin assay. The response to systemic injection could be quantified but sensitivity was not enough to detect 0.01 IU of prolactin.<BR>The injection schedule of twice a day for 2 days was most sensitive in case of intradermal administration. Several assay procedures, such as response diameter, area, dry weight of stimulated area and dry weight of mucosal epithelium, were compared, and dry weight of mucosal epithelium was found to be most sensitive and stable for prolactin bioassay.<BR>From the results mentioned above, it may be concluded that Nicolls method which measure dry weight of pigeon crop-sac mucosal epithelium is the best bioassay procedure for prolactin determination.
- 社団法人 日本内分泌学会の論文
著者
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Hiroi Masahiko
Department Of Obstetrics And Gynecology Yamagata University School Of Medicine
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HIROI MASAHIKO
Department of Obstetrics and Gynecology, Niigata University School of Medicine
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