Dextran-Coated Charcoal Technique to Make the Hormone-Free Serum as a Diluent for Standard Curve of Radioimmunoassay
スポンサーリンク
概要
- 論文の詳細を見る
Experiments were designed to make the hormone-free serum to serve for a standard curve of radioimmunoassay. The adsorption rate of hormone by dextran-coated charcoal (D. C. C.) was analysed using radioactive hormones such as 3, 5, 3 triiodothyronine (T<SUB>3</SUB>), thyroxine (T<SUB>4</SUB>), insulin, corticosterone, angiotensin I and II, ACTH, growth hormone (GH), TSH and HCG in pooled human serum. In general, the hormone of a small molecular weight was easily adsorbed into D. C. C., but the hormone of a large molecular weight needed a large amount of D. C.C. to remove hormone. Using the solution of high concentrated D. C. C.(20% charcoal and 2% dextran), T<SUB>3</SUB>, insulin, corticosterone and angiotensin I and II were adsorbed by more than 80% into a small amount of D. C. C. solution (0.1m<I>l</I>), T<SUB>4</SUB>, GH and ACTH were adsorbed by more than 80% into a relatively large amount of D. C. C. solution (1m<I>l</I>), and TSH and HCG were removed by 75% into a greater amount of D. C. C. solution (2m<I>l</I>) under 1m<I>l</I> of pooled serum. In this treatment of D. C. C. solution, serum albumin was removed slightly into charcoal.<BR>The standard curve of radioimmunoassay for TSH and GH was figured using the panhypopituitary patient serum (PPS) or PPS treated by D. C. C. The standard curve of TSH or GH using PPS did not remarkably differ from that of PPS treated by D. C. C. The hormone-free serum that was made by D. C. C. treatment is available for the standard serum for the double antibody radioimmunoassay of these hormones.
- 社団法人 日本内分泌学会の論文
著者
-
YOSHIMURA Manabu
Second Department of Internal Medicine. Kyoto Prefectural University of Medicine
-
HACHIYA TAKASHI
Second Department of Surgery, Hamamatsu University School of Medicine
-
Ochi Y
Research Institute For Production Development
-
MIYAZAKI TADAYOSHI
Second Department of Internal Medicine Kyoto Prefectural University of Medicine Kyoto
-
OCHI YUKIO
Second Department of Internal Medicine Shiga University of Medical Science
-
SHIOMI KATSUHIKO
Second Department of Internal Medicine, Kyoto Prefectural University of Medicine
-
YOSHIMURA MANABU
Second Department of Internal Medicine Kyoto Prefectural University of Medicine Kyoto
関連論文
- Hypotension and Hypothalamic Depression Produced by Intracerebroventricular Injections of GABA in Spontaneously Hypertensive Rats : THE 15th CONFERENCE ON THE PATHOGENESIS OF HYPERTENSION
- Renal Denervation Inhibits Hypothalamo-Sympathetic Nerve System in Normotensive and Hypertensive Rats : THE 14th CONFERENCE ON THE PATHOGENESIS OF HYPERTENSION
- CENTRALLY INHIBITORY EFFECT OF ADRENALLINE ON CARDIOVASCULAR AND SYMPATHETIC NERVE SYSTEM IN URETHANE ANESTHETIZED RAT
- The Role of Prostaglandins in the Regulation of Blood Pressure with Special Reference to Vascular Reactivity : SYMPOSIUM ON BLOOD PRESSURE AND VASOACTIVE SUBSTANCES
- CENTRALLY-INDUCED VASOPRESSOR RESPONSES TO OUABAIN ARE AUGMENTED IN SPONTANEOUSLY HYPERTENSIVE RATS : PROCEEDINGS OF THE 47th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY : Hypertension
- HETEROGENEITY OF VASODEPRESSOR RESPONSES BY CAPTOPRIL : Hypertension (I) : 46th Annual Scientific Meeting, Japanese Circulation Society
- Increased Receptor Binding by Bovine (b) TSH Bound to Monoclonal Antibody to bTSHβ-subunit
- Thyroid Stimulating Immunoglobulin (TSI) in Graves' Disease
- Primary Dissection of the Hepatic Artery : Report of a Case
- The Role of Brain Angiotensin II in the Mechanism of the Pressor Responses to Intracisternal Injection of Hypertonic NaCl in Urethane Anesthetized Rats : THE 11th CONFERENCE ON THE PATHOGENESIS OF HYPERTENSION
- Effect of Sodium Loading and Depletion on Vascular Reactivity and Prostacyclin Generation : IX Conference on the Pathogenesis of Hypertension
- The Origin and Clinical Significance of Thyroid Murmur
- Enhanced Vascular Reactivity to Norepinephrine in Salt-Sensitive Patients with Hypertension
- THE EFFECT OF PROSTAGLANDIN E-1 ON VASCULAR REACTIVITY AND RENINANGIOTENSIN ALDOSTERONE SYSTEM : Hypertension I : Ist Auditorium : Proceedings of the 43rd Annual Meeting of the Japanese Circulation Society, Tokyo, 1979
- An Assay for Antibodies to Thyroid Plasma Membrane Using the Isotope-labeled Protein A
- Clinical Study on Increased Serum Thyroxine-Binding Globulin in Cancerous State
- Urinary Excretion of LATS in a Patient with Gravesdisease Complicated by Nephrotic Syndrome
- Effect of Biogenic Amines on TRH-TSH System
- Failure of the Reaction of β2-Microglobulin with Staphylococcal Protein A
- Inhibitory Effect of Thyroid Blocking Antibody (TBAb) on the Thyroid Stimulatory Effect of Human Chorionic Gonadotropin (HCG) and Equine Chorionic Gonadotropin (ECG)
- An Attempt to Analyze Various Thyroid Stimulators by the Receptor Assay using hTSH Radioimmunoassay
- Changes in the Cyclic Nucleotides of Rat Thyroid, Pituitary and Plasma Caused by Methylthiouracil Treatment
- Effect of LATS, TSH and Cholera Toxin on the Thyroid Gland in Nude Mice
- Determination of Triiodothyronine in Red Blood Cells by Radioimmunoassay
- Immunological Studies on LATE-immunoglobulin by the Reaction with Staphylococcal Protein A
- Radioimmunoassay for Estimation of Thyroglobulin in Human Serum
- Stimulation of Pitutitary Glucose-6-Phosphate Dehydrogenase Activity in the Rat Associated with Increased TSH Secretion
- Dextran-Coated Charcoal Technique to Make the Hormone-Free Serum as a Diluent for Standard Curve of Radioimmunoassay
- Effect of sodium loading and depletion on cyclic nucleotides in plasma and aorta. Interaction between prostacyclin and cyclic nucleotides.
- Immunological similarity of thyroid stimulating antibody (TSAb) and thyroid blocking antibody (TBAb) with animal IgG
- Neutralization and purification of thyroid stimulating antibody (TSAb) and thyroid blocking antibody (TBAb) by heterophilic antibody to animal IgG in Graves' disease
- Effect of prostaglandin E1 on renin and aldosterone in hypertensive patients.
- Serum T3 Level in the Patients with Hyperthyroidism After Therapy
- Measurement of serum thyroxine binding prealbumin in various thyroidal states by radioimmunoassay.
- Enhanced Pressor Responses to Intracisternal Injection of Hypertonic Saline and It's Brain mechanisms in urethane Anesthetized SHR
- The Effect of Propranolol on Blood Pressure and Sympathetic Nerve Activity in the Spontaneously Hypertensive Rats
- Acute effects of captopril on the baroreflex of normotensive and spontaneously hypertensive rats.
- Role of prostaglandins in naturiuresis of perfused kidney in SHR
- Augmented central cholinergic mechanisms in spontaneously hypertensive rats. Involvement of deranged noradrenergic mechanisms in the brain.:Involvement of Deranged Noradrenergic Mechanisms in the Brain