PLASMA INSULIN ACTIVITY FOLLOWING THE ADMINISTRATION OF BZ 55 (N-SULFANYLYL-N'-n-BUTYLCARBAMIDE) IN DOGS
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(I) Determination of insulin was examined by the rat-diaphragm method, and the following results were obtained: A linear regression was observed between extraglucose uptake and log-concentration of insulin in the range of 10 to 0.01mU/kg. The minimum detectable concentration was 0.003mU/ml, slope of regression line (regression coefficient) b=0.6610, standard deviation of regression line S=0.1674, index of precision λ=0.253 (intervals between consecutive doses, 10-fold).<BR>(II) Plasma insulin activity in 14 normal fasting dogs, estimated by this method was 0.347±0.184mU/ml. Change in plasma insulin activity was observed after administration of insulin, glucose and BZ 55 (N-sulfanylyl-N'-n-butyl-carbamide) with the following results:<BR>1. After intravenous administration of 1U/kg of insulin, plasma insulin activity increased from 0.229mU/ml before to 4.655m, U/ml at 10mins. became 0.645mU/ml at 30 mins. after.<BR>2. After subcutaneous administration of 0.2-0.8U/kg of insulin the results were as follows: In case of 0.2U/kg, plasma insulin activity always remained in the normal range; but in the case of 0.6U/kg, it increased significantly after the injection. The minimum dose of insulin in subcutaneous injection which produced increase in plasma insulin activity was 0.4U/kg.<BR>3. After the intravenous injection of 1g/kg of glucose, 0.347mU/ml before the injection became 0.933mU/ml at 30mins., indicating increase.<BR>4. After intraperitoneal administration of 50mg/kg of BZ 55, 0.287-0.382mU/ml before the injection became 0.180mU/ml at 1hr. and 2hrs. After the intravenous administration of 100mg/kg, 0.471mU/ml before the injection became 0.336mU/ml at 1hr., 0.283mU/ml at 2hrs. and 0.301mU/ml at 4 hrs. In all the cases, there was no significant difference in plasma insulin activity between before and after the administration.<BR>(III) No significant effect of BZ 55 was observed in vitro experiments on the basal uptake and insulin effect by the isolated rats diaphragm.
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