尿中の無機リン酸塩の比色分析法
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概要
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Colorimetric determination of inorganic phosphate in micro amounts of human urine by molybdenum blue method was studied.<BR>Ammonium molybdate solution was mixed with Bi(NO<SUB>3</SUB>)<SUB>3</SUB> nitric acid solution and as reducing reagent, 1-amino-2-naphthol-4-sulfonic acid, Na<SUB>2</SUB>SO<SUB>3</SUB> and Na<SUB>2</SUB>S<SUB>2</SUB>O<SUB>5</SUB> were dissolved together. Those reagents remained effective for more than several months. The molybdenum blue developed was very stable and the intensity did not change at least for 2 hours.<BR>The recommended analytical procedure was as follows: 0.0025m<I>l</I> of urine adjusted to pH5.4 was taken by the use of a micro pipette. One m<I>l</I> of distilled water and 2m<I>l</I> of ammonium molybdate solution were added and shaken vigorously. Being stood for about 5 minutes, 10m<I>l</I> of distilled water and 0.5m<I>l</I> of the reducing reagent were added and absorbance was measured after 5 minutes at 730mμ by a 10mm cell. The most suitable reaction temperature was 2035°C.
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