テトラブロムフェノールフタレインエチルエステルによるホマトロピンの比色定量法

概要

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A colorimetric method for the determination of homatropine has been developed by means of solvent extraction, In the recommended procedure for preparing calibration curve, 210 ml of a standard homatropine solution (2.0×10-5M) is taken in a separatory funnel, and 5 ml of borate (0.1M) -phosphate (0.3M) buffer (pH 8.5) and 2 ml of a tetrabromophenolphthalein ethyl ester (TBPE) ethanol solution (4×10-3M) are added. It is diluted to 25 ml with water, and shaken with 10 ml of 1, 2-dichloroethane for 2 min. The extract is filtered through a dried filter paper to remove droplets of water. The absorbance is measured at 570 mμ against a reagent blank. The colored species was assumed to be [homatropine]·[H(TBPE)] (red-violet) and [quaternary ammonium ion]+·[TBPE]- (blue).